OligoCalc, a free online tool to calculate DNA and RNA sequence properties.  

Oligo Calc

Oligonucleotide Properties Calculator

Oligo Calc is a free online calculator to obtain properties of single stranded and double stranded DNA or RNA sequences. OligoCalc provides a user friendly web interface for calculating the physical properties of oligonucelotides. These properties include oligonucleotide melting temperature, sequence molecular weight, %GC content of the entered sequence and absorbance coefficients.
     


 INPUT
a sequence.
Reverse Complement Strand(5' to 3') is:
5' modification (if any) Primer nM
3' modification (if any) Salt (Na+) mM
Select molecule Absorbance at 260 nm
       
       
 

PHYSICAL CONSTANTS
Length Molecular Weight GC Content
1 ml of a sol'n with an Absorbance of at 260 nm is µM & contains µgms.
           
MELTING TEMPERATURE (TM) CALCULATIONS
Basic °C Salt Adjusted °C Nearest Neighbor °C
           
THERMODYNAMIC CONTANTS CONDITIONS: 1M NaCl at 25°C at pH 7
RlnK cal/(°K*mol) deltaH Kcal/mol
deltaG Kcal/mol deltaS cal/(°K*mol)
 
DEPRECATED HAIRPIN/SELF DIMERIZATION CALCULATIONS
Minimum base pairs required for single primer self-dimerization
Minimum base pairs required for a hairpin
 
 


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